PEP-IPC
Peptide identity, purity and net content
Identity by LC–MS molecular weight, purity by RP-HPLC-UV as area percent, and net content, on one sample.
Every sample starts with this test. Contaminant tests and packages are add-ons to it.
Specification
- Test code
- PEP-IPC
- Technique
- RP-HPLC-UV; LC–MS / ESI-MS
- Detection wavelength
- 214 nm primary (peptide bond); 280 nm secondary for Trp/Tyr-containing sequences
- Column
- C18, 5 µm, typically 4.6 × 250 mm; C4 or C8 for highly hydrophobic peptides
- Mobile phase
- Water/acetonitrile gradient with 0.1% TFA or formic acid ion pairing
- Purity calculation
- Target peak area / total integrated peak area × 100 (area percent)
- Impurity detection limit
- 0.1% of total peak area; reporting range 50%–99.9%
- Identity confirmation
- LC–MS / ESI-MS molecular weight match against theoretical mass
- Sample needed
- 100–500 µg lyophilized powder, or 1 mL of reconstituted solution
- Sample types
- lyophilized powder, reconstituted solution
- Turnaround
- 14–31 days (average)
The report includes
- Purity as area percent
- Identity: observed molecular weight against theoretical mass
- Net content
- Chromatogram and spectra
Before you order
- Label every container with the sample ID you will enter at intake.
- Read the sample submission guide for packing and shipping.